HGM2002 Poster Abstracts: 11. Genome Diversity
POSTER NO: 579
Multilocus DNA Fingerprint Analysis as a Tool for Human DNA Diversity Studies
1Elena V. Shabrova, 2Elsa K. Khusnutdinova, 3Aleksei I. Mikulich, 1Larissa A. Tarskaia, 1Svetlana A. Limborska
Hypervariable minisatellite probes have proved to be quite powerful in the describing of genetic structure of both large subdivided population and group of different ethnic populations. We have used the technique of DNA fingerprint analysis with M13 phage DNA as a probe to investigate rather heterogeneous group of 13 human populations from Eastern Europe and Asia. These populations belong to three language families: Indo-European language family (Slavonic branch: Byelorussians), Uralic language family (Finno-Ugric branch: Mari, Mordvinians, Udmurts, Komi), Altai language family (Turkic branch: Bashkirs, Tatars, Chuvashes, Yakuts). As the result of the analysis of restriction fragments patterns generated by hybridization the size matrix of all the fragments from 10,00 bp to 2,000bp was constructed. The set of individual patterns was presented as a binary matrix like 'Object-Trait'. Coefficients of differentiation for the linguistic hierarchical structure were estimated according to Lynch (1). Phylogenetic trees were constructed and multidimensional scaling was carried out on the bases of some types of the genetic distances matrices calculated from the populations profiles of fragments frequencies. Multiple correspondence analyses were applied for the treatment of binary populations matrices. Levels of population subdivision in the groups of closely related populations appeared to be quite different. In our study two territorially separated Byelorussian populations produced no regional differences in contrast to four Bashkir populations. On the whole, the correlations between our results and the linguistic affinity data and between our results and the matrix of geographical distances are not absolute.
1. Lynch M. (1990). The Similarity Index and DNA Fingerprinting. Mol. Biol. Evol. 7(5).
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