HGM2002 Poster Abstracts: 11. Genome Diversity
POSTER NO: 551
Cloning of the pigmy chimpanzee (Pan paniscus) rIGS regulatory region and its comparison with the orthological area in humans
N.S. Kupriyanova, K.K. Netchvolodov, A.S. Voronov, D.V. Shibalev, A.P. Ryskov
Previous studies with randomly cloned genomic ribosomal DNA (rDNA) and sequences derived from specific chromosomes showed that different parts of rDNA units evolve at different rates. This is true not only for comparison between transcribed and nontranscribed rDNA regions: ribosomal intergenic spacer (rIGS) comprises regions of elevated variability as well as more conservative, presumably regulatory ones. In this study, comparative analysis of a discrete part of prepromoter (regulatory) region in humans and chimpanzee (Pan paniscus) has been performed. Monkey DNA was PCR amplified with human primers specific for the region of interest, cloned into pGEM-T Easy vector and sequenced. It was shown that in spite of considerable similarity in structutal organization features of this area in humans and chimpanzee (presence of identical repeating elements and high homology regions) there exist also substantial differences revealing in point substitutions, deletions and complex structural rearrangements.
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