HGM2002 Poster Abstracts: 9. Model Organisms
POSTER NO: 497
Generation and characterization of chondrocyte specific Cre transgenic mice
Zhenming Hao, Xiao Yang, Xuan Cheng, Jiang Zhou, Cuifen Huang
A chondrocyte specific transgenic construct (pcol2A1-Cre) containing the cartilage specific type II collagen A1 promoter, the Cre recombinase gene and the polyA of human growth factor gene was generated. The 9.3kb DNA fragments were recovered from NotI digested fragments and microinjected into 323 fertilized eggs. The injected eggs were implanted into the oviduct of 14 female mice. In the 52 offspring, there were 10 mice carring the transgene identified by PCR, and the efficiency was 19.2%. The col2a1-Cre transgenic mice were crossed with a conditional gene targeting mice to check the Cre mediated recombination in multiple tissues. The results of the PCR analysis suggested that the Cre recombinase was expressed and could mediate the recombinantion between the LoxP sites in vivo. The result was further confirmed by Southern-blot.
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