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POSTER NO: 415 High-density cDNA microarray as a useful tool to identify genes specific for colon cancer liver metastasis
Jiayi Ding, James Rosinski, John Reidhaar-Olson, Barry Goggin, Sittichoke Saisanit, Mitchell Martin, Steve Ritland, Juergen Hammer Remote metastasis is the major hurdle to the successful treatment of colon cancer. To understand the molecular mechanisms in metastasis, the GeneChip Human Genome U95 chip set, which covers about 60,000 human genes and ESTs, was used to explore the genome-wide expression pattern of normal adjacent colon, colon cancer, colon cancer liver metastatic and normal liver tissues. Two-way ANOVA was used for measuring the effects of cancer status and tissue. 391 (0.65%) of 60,000 genes and ESTs showed some differential expression in metastatic tissues (p<0.05). Among them, 83 genes mapped to cellular pathways and biological processes known to be involved in carcinogenesis, including signal transduction, cell development and proliferation, cell-cell interaction, cell cycle, embryogenesis and oncogenesis. However, our experiment also revealed several genes involved in unexpected biological processes, such as neurogenesis and skeletal development. Our findings give new insights into the molecular changes involved in colon cancer metastasis, and will help to identify potential new diagnostic markers and therapeutic targets for the disease. |