POSTER NO: 383

The Analysis of Genotype Associations Distribution of Polymorphic DNA-Loci in the Serotonin Transporter Gene Depending on the Type of Disease Flow in Patients with Schizophrenia of Different Ethnic Groups

A.G. Zainullina, D.A. Romanenko, E.B. Jurjev, I.R. Valynurova, E.K. Khusnutdinova
Institute of Biochemistry and Genetics, Russia, Bashkortostan, Ufa, Pr. Octyabrya, 69

Distributions of genotype associations of VNTR (hSERT) and insertion-deletion (SLC6A4) polymorphisms of DNA-loci at the serotonin transporter gene in patients with continual and episodic types of schizophrenia were analysed. 214 Russian and Tatar patients with schizophrenia at the age of 15 - 74 were subjected to the analysis. The control group consisted of 197 persons. Genotypes 12/12, 12/10, 10/10, 12/9, 10/9 of VNTR-polymorphism and genotypes S/S, L/S, L/L of insertion-deletion polymorphism in the serotonin transporter gene were detected. The study has shown, that in all patients irrespective of disease flow there were no genotype associations 12/9, S/S and 10/9, L/S, and these genotype associations had the lowest frequencies in the control group. In controls and both Russian and tatar patients with schizophrenia very low frequencies of genotype associations 12/9, L/L, 10/10, L/S, 12/9, L/S, 10/10, S/S were determined. A significant increase of frequency of genotype association 12/12, L/S was found in the general group of patients with schizophrenia comparing with the control group (X2 = 4.01, P = 0.044) and in the group of Tatar patients with schizophrenia comparing with Tatar control group (X2 = 7.30, P = 0.003). The character of distribution of genotype associations frequencies was not significant in Russian patients with schizophrenia and in Russian control group. At the analysis carried out depending on a disease flow type a tendency to significant difference in the distribution of genotype associations of two loci - hSERT and SLC6A4 - at the serotonin transporter gene (hSERT) was found between continual and episodic types of disease flow (X2 = 16.40, P = 0.087) in patients with schizophrenia irrespective of ethnic origin. Significant increase of genotype associations 12/10, L/S (X2 = 6.54, P = 0.013) and 10/10, L/L (X2 = 6.41, P = 0.016) was found in patients with episodic type of disease flow in comparison with patients with continual type of disease flow. A significant increase of frequency of genotype associations 12/12, L/S in Tatar patients with continual type of disease flow. (X2 = 4.55, P = 0.047) and genotype associations 10/10, L/L (X2 = 4.90, P = 0.035) in Tatar patients with episodic type of disease flow in comparison with control group was shown. A significant distinction in distribution of genotype associations (X2 = 22.85, P = 0.009) was found between Tatar patients with continual type of disease flow and Tatar patients with episodic type of disease flow. A significant increase of frequencies of genotype associations 12/10, L/S (X2 = 7.03, P = 0.011) and 10/10, L/L (X2 = 11.11, P = 0.007) was found in Tatar patients with episodic type of disease flow in comparison with Tatar patients with continual type of disease flow. There were no significant distinctions among Russian patients with different types of disease flow. The obtained data testify to availability of significant distinctions in the distribution of genotype association frequencies of loci hSERT and SLC6A4 at the serotonin transporter gene among Russian and Tatar patients with continual type of disease flow in comparision with patients of the same ethnic group with episodic type of disease flow, that indicates genetic heterogeneity of the given pathology in Russian and Tatar patients.