HGM2002 Poster Abstracts: 7. Medical Genomics
POSTER NO: 373
Chromosomal imbalances in esophageal squamous cell carcinomas from north China detected by comparative genomic hybridization
1Fang Wei, 2Jiang Ni, 1Shan-Shan Wu, 1Hao Liu, 1Xin Xu, 1Ya-Ling Han, 1Yan Cai, 1Jian-Wei Zhang, 1Xian-Jun Chen, 1Hui Pang, 1Ning Lu, 2Liang Ji, 1Min Wu, 1Ming-Rong Wang
Esophageal cancer is is one of the most common tumors in human. It ranks the forth most prevalent malignancy in China. So far, the genetic events involved in esophageal cancer remain largely unknown. To identify chromosomal alterations in the disease, we performed comparative genomic hybridization (CGH) in twenty-five primary squamous cell carcinomas and three cancer cell lines of the esophagus. Results exhibited nonrandom copy number changes in chromosome DNA, with higher incidence in gain than in loss. In primary tumor tissues, the common gains were 3q(20/25), 1q(15/25), 8q (15/25), 20p(12/25), 20q(11/25), 5p(10/25), 15q(8/25), and 9q(8/25), with two minimal amplification loci mapped to chromosomal regions of 8q24(2 cases) and 11q13(7 cases). High-level amplifications were observed at 3q(8 cases), 5p(4 cases), and 8q(4 case). Losses at chromosome 3p(10/25), 4(6/25), 9p(6/25), 13q(8/25), 14q(6/25), 18p (6/25), 18q(7/25), 21q(6/25), and Xp(6/25) were identified. 10 cases showed both loss of the entire 3p and overpresentation of almost the whole 3q. No significant differences in stage or grade of tumor were found for DNA copy number changes. In the cell lines, high-level amplifications were observed on 1p and 5p. Two cell lines showed the loss of 3p. The results provided candidate regions for potential oncogenes and tumor suppressor genes related to Chinese esophageal cancer, to which further molecular studies should be addressed.
This work was supported by National Natural Science Foundation (30125026), State Key Basic Research Grant of China (G1988051205) and Chinese Hi-Tech R&D Program Grant (2001AA221151).
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