HGM2002 Poster Abstracts: 7. Medical Genomics
POSTER NO: 368
Polymorphic markers of candidate genes and genetic predisposition to ischemic heart disease in Russian patients
1Olga E. Voron'ko, 2Dmytry A. Zateyshchikov, 1Natalia M. Gorashko, 2Larissa O. Minushkina, 1Nadezhda B. Babunova, 2Anna A. Zateyshchikova, 1Ilya V. Ignatiev, 2Olga Yu. Kudryashova, 2Boris A. Sidorenko, 1Valery V. Nosikov
To study the genetic predisposition to ischemic heart disease (IHD) in Russian patients we have developed a set of polymorphic markers of several candidate genes. We have used Thr174Met and Met235Thr markers of angiotensinogen gene (AGT), I/D and G7831A markers of angiotensin I-converting enzyme gene (ACE), A1166C marker of vascular type 1 angiotensin II receptor gene (AT2R1), Glu298Asp and ecNOS4a/4b markers of endothelial NO synthase gene (NOS3), E2/E3/E4 marker of apolipoprotein E gene (APOE), Lys198Asn marker of endothelin 1 gene (EDN1), and ^677T marker of methylene tetrahydrofolate reductase gene (MTHFR). Two groups of patients have been formed: one group with ischemic heart disease (IHD+, n=172) and IHD- group (n=92) in which all patients had no clinical IHD. We have not found significant association with IHD in case of polymorphic markers of AGT, AT2R1, EDN1, APOE, MTHFR genes and I/D marker of ACE gene. The marker G7831A of ACE gene (another marker designation is PstI +/-) showed a small but statistically insignificant difference in allele and genotype frequencies. The polymorphic markers Glu298Asp and ecNOS4a/4b of NOS3 gene showed significant association with IHD. In the case of Glu298Asp marker of NOS3 gene the carriers of Glu/Glu genotype had lower risk of IHD (OR = 0.60) and the carriers of Glu/Asp genotype had higher risk of IHD (OR = 1.65). The study of allele and genotype distributions of ecNOS4a/4b marker of NOS3 gene has shown that the carriers of 4a allele and 4a/4b genotype had higher risk of IHD (OR = 1.36 and 1.75, correspondingly) whereas the carriers of 4b allele and 4b/4b genotype had lower risk of IHD (OR = 0.74 and 0.56, correspondingly). Conclusions: Our data allow us to suppose that NOS3 gene can be associated with development of IHD among Russian patients in a Moscow population.
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