POSTER NO: 329

Cytochrome P-4501A1 and Glutation S-transferase M1 genes polymorphisms in iron-deficiency anemia patients

¹A.A. Morozova, ¹G.Sh. Safuanova, ²T.V. Victorova, ²Z.M. Sultanaeva, ²E.K. Khusnutdinova
¹Bashkir State Medical University, Lenin str.-3, Ufa, Russia, ²Institute of Biochemistry and Genetics, Pr. Octyabrya-69, Ufa, Russia

Patients with iron-deficiency anemia (n=102) and community controls (n=105) were compared for rates of polymorphisms in exon 7 of the cytochrome P-4501A1 (CYP1A1) gene and homozygous deletion (0/0) of glutation S-transferase M1 (GSTM1)gene. A CYP1A1 polymorphism was detected in a HinCII polymorphism assay utilizing a primer with a single base pair mismatch. The detection of genotype 0/0 of GSTM1 gene was performed by absent of amplification fragment in 271 p.n. The increased frequency of Val allele of CYP1A1 gene in iron- deficiency anemia patients (10,21%)showed significant difference from that in healthy controls (4,29%, p<0,05, OR=3,17). The frequency of Ile/Val genotypes of CYP1A1 gene in iron-deficiency anemia patients (16,12%)was also statisticaly significant compared with controls (5,71%, p<0,05, OR=2,54). The frequency of the GSTM1 0/0 genotypes was 37,8% in iron-deficiency anemia patients and 50,6% in control group (p>0,05). These results lead us to suppose that mutant CYP1A1 gene is a predisposition gene to iron-deficiency anemia, and GSTM1 0/0 genotype was not linked with this disease.