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POSTER NO: 225 Gene expression profiling in kidney of zebrafish's hematopoietic tissue
Huai-Dong Song The zebrafish, Danio rerio, was originally envisioned as a model to bridge the gap between fly/worm and mouse/human for understanding embryonic development. Recent studies have indicated that the zebrafish has a great potential to serve as a model for human disease, particularly for the study of hematopoiesis. The kidney is the hematopoietic tissue of zebrafish, so the gene expression profiling in the zebrafish kidney was studied by generating a large amount of expressed sequence tags (ESTs). Totally, 7,199 sequences with good quality were obtained from 10,239 clones (70.4%) form cDNA library of the zabrafish kidney. After bioinformatics analysis, 686 ESTs homologous to Alu, L1, and other repetitive elements and mtDNA were put aside. The remaining 6,965 ESTs could be assembled into 2,808 clusters. Of these, 39.6% matched to zebrafish known genes or human orthologus, 19.2% matched to zebrafish ESTs, and 41.2% showed no match to any ESTs or known genes. A total of 1,111 unique known genes were used to analyze the gene expression patterns in the kidney of zebrafish hematopoietic tissue. These were categorized into 8 categories according the basis of gene function. The largest class of genes represented those involved in gene/protein expression. Some of genes involving in the hematopoiesis were expressed in the zebrafish's kidney. All of these data may contribute to understanding of the function of the zebrafish's kidney. |