POSTER NO: 212

Simultaneous characterization of GSTM1 and NAT2 polymorphisms by polymerase chain reaction in petrochemical workers from Bashkortostan

¹Olga Makarova, ²Lylia Karimova, ²Galina Gimranova, ²Svetlana Tchurmantaeva, ¹Tatiyana Victorova
¹Institute of Biochemistry and Genetics, Ufa, Russian Federation, ²Institute of occupational medicine and human ecology, Ufa, Russian Federation

The genetic polymorphism of glutathion-S-transferase M1 (GSTM1) and arylamine-N-acetyltrasferase (NAT2) genes responsible for xenobiotic conjugating enzymes of Phase II detoxification system were studied by PCR method in 165 petrochemical workers (61,2% men, 32,8% women) professionally exposed to isopentan, stirol and other aromatic hydrocarbons. The control group consisted of 90 healthy individuals living in Bashkir Republic. The analysis of the GSTM1 gene polymorphism has demonstrated that the frequency of GSTM1 gene deletion was significantly lower in workers (30% versus 54,4% in control, x2=11,82; p=0,002). The proportion of NAT2 heterozygotes (S/R) was higher in the investigated group (47,54%) compared to controls (35,59%), these differences were significant (x2=11,17; p=0,002). The combination of GSTM1 (+/+) and NAT2 (S/R) genotypes has been recorded with the maximum frequency (32,79%) in the group of petrochemical workers. The highest frequency of GSTM1 (+/+), NAT2 (R/R) genotype combination was in the control group - 24,59%.