HGM2002 Poster Abstracts: 4. Functional Genomics
POSTER NO: 182
Identification for metastatic Genes in oral carcinoma cells by cDNA Array
Wan T. Chen, Dan Huang, Wei L. Qiu, Rong G. He
Objective: To investigate the molecular event and metastasis related gene between ACC-2 and ACC-M cells in adenoid cystic carcinoma. Material and Methods: Adenoid cystic carcinoma cell line ACC-2 and selection of adenoic cystic carcinoma cell clone highly metastatic to the lung (ACC-M) were used in this study. ACC-2 and ACC-M cells were cultured and collected. Total RNA was extracted using standard Trizol RNA isolation protocol. The poly-A mRNA was purified and labeled in reverse transcription using M-MLV reverse transcriptase in the presence of Easytides deoxyadenosin 5' triphosphate [alpha-33p]. A cDNA array was assembled with 7675 EST clones which represent same number of independent unigenes. Prepared nylon membranes were hybridized with the [alpha 33p]-dATP labeled mRNA from ACC-2 and ACC-M cells. Membranes were exposed to phosphor screen. Results: According to each gene's ratio of expression level, there were 36 genes which were up-regulated with the ratio over 3.0, down-regulated with the ratio below 0.33(1/3.0=0.33). Conclusions: Significant different expression alteration pattern between ACC-2 and ACC-M by cDNA array. The Difference genes lie in signal pathway, tumor antigen, immune molecular and some unknown genes.
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