POSTER NO: 182

Identification for metastatic Genes in oral carcinoma cells by cDNA Array

Wan T. Chen, Dan Huang, Wei L. Qiu, Rong G. He
Afilliated Ninth People's Hospital, Shanghai Second Medical University, 639 Zhizaoju Road, Shanghai 200011, P.R. of China

Objective: To investigate the molecular event and metastasis related gene between ACC-2 and ACC-M cells in adenoid cystic carcinoma. Material and Methods: Adenoid cystic carcinoma cell line ACC-2 and selection of adenoic cystic carcinoma cell clone highly metastatic to the lung (ACC-M) were used in this study. ACC-2 and ACC-M cells were cultured and collected. Total RNA was extracted using standard Trizol RNA isolation protocol. The poly-A mRNA was purified and labeled in reverse transcription using M-MLV reverse transcriptase in the presence of Easytides deoxyadenosin 5' triphosphate [alpha-33p]. A cDNA array was assembled with 7675 EST clones which represent same number of independent unigenes. Prepared nylon membranes were hybridized with the [alpha 33p]-dATP labeled mRNA from ACC-2 and ACC-M cells. Membranes were exposed to phosphor screen. Results: According to each gene's ratio of expression level, there were 36 genes which were up-regulated with the ratio over 3.0, down-regulated with the ratio below 0.33(1/3.0=0.33). Conclusions: Significant different expression alteration pattern between ACC-2 and ACC-M by cDNA array. The Difference genes lie in signal pathway, tumor antigen, immune molecular and some unknown genes.