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POSTER NO: 1 Sanger Chromosome Gene Sets: High quality manual annotation
Jennifer Ashurst, Michele Clamp, James Gilbert, Stephen Kennan, Andrew King, Charles Steward, Laurens Wilming, Tim Hubbard The value of a genome is only as good as its annotation. At the Sanger Institute, we are providing high quality manual curation in addition to automated predictions provided by Ensembl. Finished genomic sequence is analysed on a clone by clone basis using a combination of similarity searches against DNA and protein databases as well as a series of ab initio gene predictions (GENSCAN, FGENESH). In addition, comparative analysis using vertebrate datasets such as the Riken mouse cDNAs and Genescope Tetraodon Ecores are used for novel gene discovery. Currently over 10% of the human genome has been annotated by the Human analysis team. This includes two complete chromosomes, 20 and 22, and is in collaboration with the Chromosome Project Teams. These gene sets are publically available from http://www.sanger.ac.uk/HGP/ . It took the groups over six months to identify 727 genes on Chromosome 20. Improvements in annotation tools and automated analysis methods should help the public consortium achieve its goal of high quality annotation of every Human Chromosome by 2003. |